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Biotechnology (Wet Lab) Training and Project in Bio-EGICORE with BDRC

Training is the acquisition of knowledge, skills, and competencies as a result of the teaching of vocational or practical skills and knowledge that relate to specific useful competencies.

Bio-EGICORE and BDRC are highly commited to spread the technical knowledge by different type of industrial training, projects, dissertation work, short term programs, weekend classes (e.g. Seminars, Conferences, Symposium, Workshops & Quizes etc.) and Online Training Programs.

 

Biotechnology (Wet Lab) Training and Project Program

on Our Campus

Registration Fees Rs. 500/- Including Course Fees

Courses Available for 7 Days (Fees: Rs. 3,500/-)

Course Code Course Name & Structure
Module BT-A1
PCR Techniques*

Content: Isolation of Human DNA, Gel electrophoresis, A lecture on primer designing, Amplification of gene of interest and Confirmation of amplified product on agarose gel

Module BT-A2
Gel Electrophoresis Techniques

Content: Isolation of DNA from natural source, Agarose gel electrophoresis, SDS-Polyacrylamide gel electrophoresis, Native-Polyacrylamide gel electrophoresis and Immuno eletrophoresis

Module BT-A3
DNA Fingerprinting

Content: DNA Fingerprinting as a tool in Forensic investigation, Instrumentation for DNA fingerprinting, Isolation of DNA from blood sample, Evidence collection procedures & preservation, Isolation of DNA from cheek cells, Agarose gel Electrophoresis, Restriction enzymes in the field of DNA Fingerprinting, Restriction digestion of DNA, Separation of Fragments by AGE and Disputed Paternity establishment basics-The STR Analysis.

Module BT-A4
Enzymology & Microbial Biotechnology

Content: Basics of Enzyme technology and importance of biocatalysts, Isolation of micro-organisms from soil by serial dilution, Study of Colony morphology, Pure culture preparation, Screening for enzyme production, Identification of bacteria by Gram Staining, In vitro production of enzymes, Salt precipitation of enzymes, Enzyme assay and Immobilization of Enzyme.

Courses Available for 15 Days (Fees: Rs. 4,500/-)

Course Code Course Name & Structure
Module BT-B2
Recombinant DNA Technology

Content: An introduction to Recombinant DNA Technology, Instrumentation in Biotechnology and Molecular Biology, Isolation of DNA from fruit, Isolation of DNA from Plant source by SDS and C-TAB method, Industrial Isolation and purification of Genomic DNA from yeast, Isolation and purification of Genomic DNA from Prokaryotes, Isolation of Plasmid DNA from prokaryotes, Purification of DNA and Separation of DNA by Gel electrophoresis technique.

Module BT-B2
Immunotechnology*

Content: Basics of cells and organs involved in Immune system, Basic concepts of Antigen-Antibody interactions, Immuno diffusion principles, ODD test, RID test, HCG test (Pregnancy test), Blood Grouping and Rh typing, HIV Tridot test & Widal test.

Courses Available for 30 Days Project/Training (Fees: Rs. 6,500/-)
For Project Alongwith Training (Fees Rs. 8,500/-)

Course Code Course Name & Structure
Module BT-C1
Basic Recombinant DNA Technology

Content: An introduction to Recombinant DNA Technology, Instrumentation in Biotechnology and Molecular Biology, Isolation of DNA from fruit, Isolation of DNA from Plant source by SDS and C-TAB method, Industrial Isolation and purification of Genomic DNA from yeast, Isolation and purification of Genomic DNA from Prokaryotes, Isolation of Plasmid DNA from prokaryotes, Purification of DNA, Separation of DNA by Gel electrophoresis technique, Restriction enzymes in the field of Recombinant DNA Technology, Restriction digestion, Agarose gel Electrophoresis, Quantitative estimation of DNA, Competent cell Preparation, Transformation, Screening of transformed cells and DNA sequencing.

Module BT-C2
Basic Enzymology and Microbial Technology

Content: Basics of Enzyme technology and importance of biocatalysts in different, metabolic pathways, Isolation of micro-organisms from soil by serial dilution, Study of Colony morphology, Pure culture preparation, Sterilization techniques, Screening for enzyme production, Identification of bacteria by Gram Staining, Biochemical characterization, In vitro production of enzymes, Salt precipitation of enzymes, Enzyme assay, Quantitative estimation of Enzymes, Kinetic study of enzymes
i) Effect of Activator
ii) Effect of Inhibitor
and Immobilization of Enzyme.

Module BT-C3
Food Microbiology

Content: Isolation of microbes from fermented foods, Study of Aflatoxin producing fungi- Aspergillus flavus, Isolation of microbes from infected fruits and vegetables, Isolation of microbes from dairy products, Production and Estimation of Citric acid from Aspergillus niger, Quality analysis of water by MPN test Production of Bio-yoghurt, Quality analysis of canned food, Quality analysis of milk, Production and estimation of Alcohol from Yeast and Antimicrobial compounds in food.

Module BT-C4
Agricultural Microbiology

Content: Isolation of Cellulose degrading Microbes, Isolation of Pectin degrading Microbes, Isolation of Starch degrading Microbes Isolation of Lignin degrading Microbes, Isolation of Rhizobium from Root Nodules of legumes, Isolation of non-symbiotic Azotobacter from Soil, Isolation of Associative Nitrogen fixer - Azospirillum, Demonstration of Nitrate reduction by Bacteria, Assay of Biofertilizers, Estimation of Protein and carbohydrate content of mushroom and Production of Synthetic Seeds.

Courses Available for 45 Days Project/Training (Fees: Rs. 8,000/-)
For Project Alongwith Training (Fees Rs. 10,000/-)

Course Code Course Name & Structure
Module BT-D1
DNA Forensics

Content: Introduction of Biotechnology (Wet Lab), Instrumentation for DNA Forensics, Isolation of DNA from blood sample, Crime Scene basics and Types of evidences in various scenes, Microscopic examination of Blood and hair, Evidence collection kits, procedures & preservation, Isolation of DNA from cheek cells, Crime Scene investigation procedures, Isolation of DNA from hair root, Purification of DNA, Agarose gel Electrophoresis, DNA Fingerprinting as a tool in Forensic investigation Restriction enzymes in the field of DNA Fingerprinting, Restriction digestion of DNA, Separation of Fragments by AGE, Procedure for Primer designing and applications, Application of PCR technology in Forensic DNA profiling, Amplification of variable regions using PCR, Agarose gel Electrophoresis for amplified products, Disputed Paternity establishment basics-The STR Analysis and Making and understanding postmortem reports.

Module BT-D2
Recombinant DNA & PCR Technology

Content: Introduction of Biotechnology (Wet Lab), An introduction to Recombinant DNA Technology, Instrumentation in Biotechnology and Molecular Biology, Isolation of DNA from fruit, Isolation of DNA from Plant source by SDS and C-TAB method, Industrial Isolation and purification of Genomic DNA from yeast, Isolation and purification of Genomic DNA from Prokaryotes, Isolation of Plasmid DNA from prokaryotes, Purification of DNA, Separation of DNA by Gel electrophoresis technique, Southern Blotting and its application spectrum, Restriction enzymes in the field of Recombinant DNA Technology, Restriction digestion, Agarose gel Electrophoresis, Quantitative estimation of DNA, SDS-PAGE, a molecular separation technique, Competent cell Preparation, Transformation, Screening of transformed cells, Primer designing and applications, PCR Basics, Amplification of gene of interest, Stem cells therapeutics in Cancer & DNA sequencing.

Module BT-D3
Industrial Enzymology & Microbial Biotechnology

Content: Introduction of Biotechnology (Wet Lab), Basics of Enzyme technology and importance of biocatalysts in different metabolic pathways, Isolation of micro-organisms from soil by serial dilution, Study of Colony morphology, Pure culture preparation, Sterilization techniques, Screening for enzyme production, Identification of bacteria by Gram Staining, Biochemical characterization, In vitro production of enzymes, Enzyme assay, Purification of enzymes:
i) Salt precipitation
ii) Dialysis
Assay for purified enzyme, Quantitative estimation of Enzymes, Kinetic study of enzymes:
i) Effect of Activator
ii) Effect of Inhibitor
iii) Effect of Substrate Concentration
iv) Effect of temperature
Immobilization of Enzyme, Isolation of Genomic DNA from Prokaryotes, Isolation of Plasmid DNA from prokaryotes & Separation of DNA by Gel electrophoresis technique.

Courses Available for 2 Months (Fees: Rs. 10,000/-)
For Project Alongwith Training (Fees Rs. 12,000/-)

Course Code Course Name & Structure
Module BT-E1
Elective Enzymology & Microbial Biotechnology

Content: Introduction of Biotechnology (Wet Lab), Basics of Enzyme technology and importance of biocatalysts in different metabolic pathways, Isolation of micro-organisms (Bacteria & Fungi) from soil by serial dilution, Study of Colony morphology (Bacteria), Pure culture preparation (Bacteria & Fungi), Sterilization techniques, Morphological characterization of fungi, Screening for enzyme production
• Starch hydrolysis
• Casein hydrolysis
Identification of bacteria by Gram Staining, Identification of fungi by Lactophenol cotton blue staining, Biochemical characterization, Antibiotic sensitivity test for bacteria, In vitro production of enzymes, Enzyme assay, Purification of enzymes
i) Salt precipitation
ii) Dialysis
iii) Ion exchange chromatography
Assay for purified enzyme, Quantitative estimation of Enzymes, Kinetic study of enzymes
• Effect of Activator
• Effect of Inhibitor
• Effect of Substrate Concentration
• Effect of temperature
• Effect of pH
Molecular weight determination by SDS-PAGE, Immobilization of Enzyme, Isolation of Genomic DNA from Prokaryotes, Isolation of Plasmid DNA from prokaryotes, Estimation of DNA & Separation of DNA by Gel electrophoresis technique .

Module BT-E2
Elective Recombinant DNA Technology

Content: Introduction of Biotechnology (Wet Lab), An introduction to Recombinant DNA Technology, Instrumentation in Biotechnology and Molecular Biology, Isolation of DNA from fruit, Isolation of DNA from Plant source by SDS and C-TAB method, Industrial Isolation and purification of Genomic DNA from yeast, Isolation and purification of Genomic DNA from Prokaryotes, Isolation of Plasmid DNA from prokaryotes, Purification of DNA, Separation of DNA by Gel electrophoresis technique, Southern Blotting and its application spectrum, Elution of inserts from gel, Restriction enzymes in the field of Recombinant DNA Technology, Restriction digestion, Ligation Agarose gel Electrophoresis, Quantitative estimation of DNA, Extraction of proteins, Quantification of proteins, SDS-PAGE, a molecular separation technique, Western Blotting, Competent cell Preparation, Transformation, Screening of transformed cells, Primer designing and applications, PCR Basics, Amplification of gene of interest, Stem cells therapeutics in Cancer & DNA sequencing.

Courses Available for 3 Months (Fees: Rs. 14,000/-)
For Project Alongwith Training (Fees Rs. 16,000/-)

Course Code Course Name & Structure
Module BT-F1
Advance Recombinant DNA Technology & Microbial Biotechnology

Content: An introduction to Recombinant DNA Technology, Instrumentation in Biotechnology and Molecular Biology, Isolation of DNA from fruit, Isolation of DNA from Plant source by SDS and C-TAB method, Industrial Isolation and purification of Genomic DNA from yeast, Isolation and purification of Genomic DNA from Prokaryotes, Isolation of Plasmid DNA from prokaryotes, Purification of DNA, Separation of DNA by Gel electrophoresis technique, Southern Blotting and its application spectrum Restriction enzymes in the field of Recombinant DNA Technology, Restriction digestion, Agarose gel Electrophoresis, Quantitative estimation of DNA, SDS-PAGE, a molecular separation technique, Competent cell Preparation, Transformation, Screening of transformed cells, Primer designing and applications, PCR Basics, Amplification of gene of interest, Stem cells therapeutics in Cancer, DNA sequencing, Basics of Enzyme technology and importance of biocatalysts in different, metabolic pathways, Isolation of micro-organisms from soil by serial dilution, Study of Colony morphology, Pure culture preparation, Sterilization techniques, Screening for enzyme production, Identification of bacteria by Gram Staining, Biochemical characterization,In vitro production of enzymes, Enzyme assay, Purification of enzymes
i) Salt precipitation
ii) Dialysis
Assay for purified enzyme, Quantitative estimation of Enzymes, Kinetic study of enzymes
i) Effect of Activator
ii) Effect of Inhibitor
iii) Effect of Substrate Concentration
iv) Effect of temperature Immobilization of Enzyme and Project. .

For Projects/Dissertation/Industrial Training/Diploma Available in Above Mentioned Topics.

  • 4.5 Months (Fees: Rs. 21,500/-)
  • 6 Months (Fees: Rs. 25,500/-)

 

 

How to apply:

Bank Draft/At Par Cheque must be drawn in favor of “en-GENE-ier’s Core Technology Services” payable at Lucknow along with the registration form. Please Take care of  the spellings.

* These courses are available in Hyderabad branch only.

 

How To Find Us

1/137, Vineet Khand, Gomtinagar, Lucknow-226010, U.P. India. 

 

Mob: +91-8563054945/
+91-9140020061

Email:  bbsbuddingbio@gmail.com

Web: www.buddingbio.com

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